Androgen regulation of gene expression in human meibomian gland and conjunctival epithelial cells.

ABSTRACT:

Purpose

Androgens exert a significant influence on the structure, function and/or pathophysiology of the meibomian gland and conjunctiva. We sought to determine whether this hormone action involves the regulation of epithelial cell gene expression in these tissues.

Methods

Immortalized human meibomian gland and conjunctival epithelial cells were treated with placebo or dihydrotestosterone (DHT) and processed for molecular biologic procedures. Gene expression was evaluated with BeadChips and data were analyzed with bioinformatic and statistical software.

Results

Androgen treatment significantly influenced the expression of approximately 3,000 genes in immortalized human meibomian gland and conjunctival epithelial cells. The nature of DHT action on gene activity was pre

SUBMITTER: Khandelwal P 

PROVIDER: S-EPMC3351406 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature