Identification of GABA receptors in chick cornea.

ABSTRACT:

Purpose

The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea. The aim of this study was to determine if GABA receptors are located in chick cornea.

Methods

Corneal tissues were collected from 25, 12-day-old chicks. Real time PCR, western blot, and immunohistochemistry were used to determine whether alpha(1) GABA(A), GABA(B), and rho(1) GABA(C) receptors were expressed and located in chick cornea.

Results

Corneal tissue was positive for alpha(1) GABA(A) and rho(1) GABA(C) recepto

SUBMITTER: Cheng ZY 

PROVIDER: S-EPMC3351410 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature