Transgene expression and local tissue distribution of naked and polymer-condensed plasmid DNA after intradermal administration in mice.

ABSTRACT: DNA vaccination using cationic polymers as carriers has the potential to be a very powerful method of immunotherapy, but typical immune responses generated have been less than robust. To better understand the details of DNA vaccine delivery in vivo, we prepared polymer/DNA complexes using three structurally distinct cationic polymers and fluorescently labeled plasmid DNA and injected them intradermally into mice. We analyzed transgene expression (luciferase) and the local tissue distribution of the labeled plasmid at the injection site at various time points (from hours to days). Comparable numbers of luciferase expressing cells were observed in the skin of mice receiving naked plasmid or polyplexes one day after transfection. At day 4, however, the polyplexes appeared to result in more tr

SUBMITTER: Palumbo RN 

PROVIDER: S-EPMC3351500 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature