B-cell tolerance defects in the B6.Aec1/2 mouse model of Sjogren's syndrome.

ABSTRACT:

Purpose

Primary Sjögren's syndrome (SjS) is an autoimmune disorder characterized by lymphocytic infiltration of the salivary and lacrimal glands, B-cell clonal expansions and an increased risk of lymphoma. In order to understand the role of B cells in this disorder, the antibody repertoire and B-cell maturation were studied in a mouse model of SjS called B6.Aec1/2.

Methods

B6.Aec1/2 serum was analyzed for antibodies by ELISA and immunoprecipitation, B-cell development by flow cytometry, and antibody gene rearrangements by CDR3 spectratyping and quantitative PCR. In order to test the functional consequences of the observed defects, B6.Aec1/2 mice were crossed with anti-dsDNA antibody heavy chain knock-in mice (B6.56R).

Results

B6.Aec1/2 mice exhibit B-cell clonal expan

SUBMITTER: Meng W 

PROVIDER: S-EPMC3351565 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature