Side-stream smoking reduces intestinal inflammation and increases expression of tight junction proteins.

ABSTRACT:

Aim

To investigate the effect of side-stream smoking on gut microflora composition, intestinal inflammation and expression of tight junction proteins.

Methods

C57BL/6 mice were exposed to side-stream cigarette smoking for one hour daily over eight weeks. Cecal contents were collected for microbial composition analysis. Large intestine was collected for immunoblotting and quantitative reverse transcriptase polymerase chain reaction analyses of the inflammatory pathway and tight junction proteins.

Results

Side-stream smoking induced significant changes in the gut microbiota with increased mouse intestinal bacteria, Clostridium but decreased Fermicutes (Lactoccoci and Ruminococcus), Enterobacteriaceae family and Segmented filamentous baceteria compared to the control mic

SUBMITTER: Wang H 

PROVIDER: S-EPMC3351767 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature