Allelic imbalance in Drosophila hybrid heads: exons, isoforms, and evolution.

ABSTRACT: Unraveling how regulatory divergence contributes to species differences and adaptation requires identifying functional variants from among millions of genetic differences. Analysis of allelic imbalance (AI) reveals functional genetic differences in cis regulation and has demonstrated differences in cis regulation within and between species. Regulatory mechanisms are often highly conserved, yet differences between species in gene expression are extensive. What evolutionary forces explain widespread divergence in cis regulation? AI was assessed in Drosophila melanogaster-Drosophila simulans hybrid female heads using RNA-seq technology. Mapping bias was virtually eliminated by using genotype-specific references. Allele representation in DNA sequencing was used as a prior in a novel Bayesian m

SUBMITTER: Graze RM 

PROVIDER: S-EPMC3351786 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature