Quantifying apoprotein synthesis in rodents: coupling LC-MS/MS analyses with the administration of labeled water.

ABSTRACT: Stable isotope tracer studies of apoprotein flux in rodent models present difficulties as they require working with small volumes of plasma. We demonstrate the ability to measure apoprotein flux by administering either (2)H- or (18)O-labeled water to mice and then subjecting samples to LC-MS/MS analyses; we were able to simultaneously determine the labeling of several proteolytic peptides representing multiple apoproteins. Consistent with relative differences reported in the literature regarding apoprotein flux in humans, we found that the fractional synthetic rate of apoB is greater than apoA1 in mice. In addition, the method is suitable for quantifying acute changes in protein flux: we observed a stimulation of apoB production in mice following an intravenous injection of Intralipid and

SUBMITTER: Zhou H 

PROVIDER: S-EPMC3351829 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature