Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.

ABSTRACT:

Background

The secretory leukocyte protease inhibitor (SLPI) exerts wide ranging effects on inflammatory pathways and is upregulated in EAE but the biological role of SLPI in EAE, an animal model of multiple sclerosis is unknown

Methods

To investigate the pathophysiological effects of SLPI within EAE, we induced SLPI-neutralizing antibodies in mice and rats to determine the clinical severity of the disease. In addition we studied the effects of SLPI on the anti-inflammatory cytokine TGF-β.

Results

The induction of SLPI neutralizing antibodies resulted in a milder disease course in mouse and rat EAE. SLPI neutralization was associated with increased serum levels of TGF-β and increased numbers of FoxP3+ CD4+ T cells in lymph nodes. In vitro, the addition of SLPI signifi

SUBMITTER: Muller AM 

PROVIDER: S-EPMC3352067 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature