Effects of doxycycline on gene expression in Wolbachia and Brugia malayi adult female worms in vivo.

ABSTRACT:

Background

Most filarial nematodes contain Wolbachia symbionts. The purpose of this study was to examine the effects of doxycycline on gene expression in Wolbachia and adult female Brugia malayi.

Methods

Brugia malayi infected gerbils were treated with doxycycline for 6-weeks. This treatment largely cleared Wolbachia and arrested worm reproduction. RNA recovered from treated and control female worms was labeled by random priming and hybridized to the Version 2- filarial microarray to obtain expression profiles.

Results and discussion

Results showed significant changes in expression for 200 Wolbachia (29% of Wolbachia genes with expression signals in untreated worms) and 546 B. malayi array elements after treatment. These elements correspond to known genes and also to

SUBMITTER: Rao RU 

PROVIDER: S-EPMC3352068 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature