Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers.

ABSTRACT:

Background

Field pea (Pisum sativum L.) and faba bean (Vicia faba L.) are cool-season grain legume species that provide rich sources of food for humans and fodder for livestock. To date, both species have been relative 'genomic orphans' due to limited availability of genetic and genomic information. A significant enrichment of genomic resources is consequently required in order to understand the genetic architecture of important agronomic traits, and to support germplasm enhancement, genetic diversity, population structure and demographic studies.

Results

cDNA samples obtained from various tissue types of specific field pea and faba bean genotypes were sequenced using 454 Roche GS FLX Titanium technology. A total of 720,324 and 304,680 reads for field pea and faba bean, resp

SUBMITTER: Kaur S 

PROVIDER: S-EPMC3352077 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature