Detection of promoter hypermethylation of the CpG island of E-cadherin in gastric cardiac adenocarcinoma.

ABSTRACT:

Aim

Abnormal hypermethylation of CpG islands associated with tumor suppressor genes can lead to transcriptional silencing in neoplasia. The aim of this study was to investigate the promoter methylation and expression of E-cadherin gene in gastric cardiac adenocarcinoma (GCA).

Methods

A nested MSP approach, immunohistochemistry method and RT-PCR were used respectively to examine the methylation status of the 5' CpG island of E-cadherin, its protein expression and mRNA expression in tumors and corresponding normal tissues.

Results

E-cadherin was methylated in 63 of 92 (68.5%) tumor specimens, which was significantly higher than that in corresponding normal tissues (P<0.001). Methylation frequencies of stage III and IV tumor tissues was significantly higher than that in

SUBMITTER: Guo W 

PROVIDER: S-EPMC3352230 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature