North American West Nile virus genotype isolates demonstrate differential replicative capacities in response to temperature.

ABSTRACT: The presence of West Nile virus (WNV) was first documented in California, USA, during the summer of 2003, and subsequently the virus has become endemic throughout the state. Sequence analysis has demonstrated that the circulating strains are representative of the North American (WN02) genotype that has displaced the East Coast genotype (NY99). A recent study has indicated that enhanced vector competence at elevated temperatures may have played a role in the displacement of the East Coast genotype by WN02. In the current study, four WN02 strains from California, including an initial 2003 isolate (COAV997), were compared to strain NY99 in growth curve assays in mosquito and duck embryonic fibroblast (DEF) cell lines at differing, biologically relevant temperatures to assess the relative temp

SUBMITTER: Andrade CC 

PROVIDER: S-EPMC3352365 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature