Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.

ABSTRACT:

Background

Prostate-specific antigen (PSA) screening, although common, has recently been called into question. To find prostate cancer (PCa) diagnostic biomarkers that can make up for the defects of PSA, we compared the secretomes of several benign and PCa cell lines, selected candidate molecules, and then confirmed their clinical value.

Methodology/principal findings

We first identified extracellular proteins by two-dimensional gel electrophoresis (2-DE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification. We then validated the secreted proteins on a cellular level, and finally determined whether they could be used as PCa diagnostic biomarkers using prostate tissue and serum specimens of Chinese volunteers by immunohistostaining and sandwic

SUBMITTER: Qian X 

PROVIDER: S-EPMC3352876 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature