Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome.

ABSTRACT: Campylobacter jejuni is the most common bacterial cause of foodborne disease in the developed world. Its general physiology and biochemistry, as well as the mechanisms enabling it to colonize and cause disease in various hosts, are not well understood, and new approaches are required to understand its basic biology. High-throughput sequencing technologies provide unprecedented opportunities for functional genomic research. Recent studies have shown that direct Illumina sequencing of cDNA (RNA-seq) is a useful technique for the quantitative and qualitative examination of transcriptomes. In this study we report RNA-seq analyses of the transcriptomes of C. jejuni (NCTC11168) and its rpoN mutant. This has allowed the identification of hitherto unknown transcriptional units, and further defines

SUBMITTER: Chaudhuri RR 

PROVIDER: S-EPMC3353397 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature