Multicolor super-resolution fluorescence imaging via multi-parameter fluorophore detection.

ABSTRACT: Understanding the complexity of the cellular environment will benefit from the ability to unambiguously resolve multiple cellular components, simultaneously and with nanometer-scale spatial resolution. Multicolor super-resolution fluorescence microscopy techniques have been developed to achieve this goal, yet challenges remain in terms of the number of targets that can be simultaneously imaged and the crosstalk between color channels. Herein, we demonstrate multicolor stochastic optical reconstruction microscopy (STORM) based on a multi-parameter detection strategy, which uses both the fluorescence activation wavelength and the emission color to discriminate between photo-activatable fluorescent probes. First, we obtained two-color super-resolution images using the near-infrared cyanine dy

SUBMITTER: Bates M 

PROVIDER: S-EPMC3353744 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature