Targeting of the human coagulation factor IX gene at rDNA locus of human embryonic stem cells.

ABSTRACT:

Background

Genetic modification is a prerequisite to realizing the full potential of human embryonic stem cells (hESCs) in human genetic research and regenerative medicine. Unfortunately, the random integration methods that have been the primary techniques used keep creating problems, and the primary alternative method, gene targeting, has been effective in manipulating mouse embryonic stem cells (mESCs) but poorly in hESCs.

Methodology/principal findings

Human ribosomal DNA (rDNA) repeats are clustered on the short arm of acrocentric chromosomes. They consist of approximately 400 copies of the 45S pre-RNA (rRNA) gene per haploid. In the present study, we targeted a physiological gene, human coagulation factor IX, into the rDNA locus of hESCs via homologous recombination. Th

SUBMITTER: Liu X 

PROVIDER: S-EPMC3353886 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature