Using rapid indicators for Enterococcus to assess the risk of illness after exposure to urban runoff contaminated marine water.

ABSTRACT:

Background

Traditional fecal indicator bacteria (FIB) measurement is too slow (>18 h) for timely swimmer warnings.

Objectives

Assess relationship of rapid indicator methods (qPCR) to illness at a marine beach impacted by urban runoff.

Methods

We measured baseline and two-week health in 9525 individuals visiting Doheny Beach 2007-08. Illness rates were compared (swimmers vs. non-swimmers). FIB measured by traditional (Enterococcus spp. by EPA Method 1600 or Enterolert™, fecal coliforms, total coliforms) and three rapid qPCR assays for Enterococcus spp. (Taqman, Scorpion-1, Scorpion-2) were compared to health. Primary bacterial source was a creek flowing untreated into ocean; the creek did not reach the ocean when a sand berm formed. This provided a natural experiment f

SUBMITTER: Colford JM 

PROVIDER: S-EPMC3354759 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature