BRITER: a BMP responsive osteoblast reporter cell line.

ABSTRACT:

Background

BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute such screens.

Methodology/principal findings

We have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifi

SUBMITTER: Yadav PS 

PROVIDER: S-EPMC3354957 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature