Osmolyte-induced perturbations of hydrogen bonding between hydration layer waters: correlation with protein conformational changes.

ABSTRACT: Gadolinium vibronic sideband luminescence spectroscopy (GVSBLS) is used to probe osmolyte-induced changes in the hydrogen bond strength between first and second shell waters on the surface of free Gd(3+) and Gd(3+) coordinated to EDTA and to structured calcium binding peptides in solution. In parallel, Raman is used to probe the corresponding impact of the same set of osmolytes on hydrogen bonding among waters in the bulk phase. Increasing concentration of added urea is observed to progressively weaken the hydrogen bonding within the hydration layer but has minimal observed impact on bulk water. In contrast, polyols are observed to enhance hydrogen bonding in both the hydration layer and the bulk with the amplitude being polyol dependent with trehalose being more effective than sucrose, gl

SUBMITTER: Guo F 

PROVIDER: S-EPMC3354986 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature