RNA-Seq reveals different mRNA abundance of transporters and their alternative transcript isoforms during liver development.

ABSTRACT: During development, the maturation of liver transporters is essential for chemical elimination in newborns and children. One cannot compare the real abundance of transcripts by conventional messenger RNA (mRNA) profiling methods; in comparison, RNA-Seq provides a "true quantification" of transcript counts and an unbiased detection of novel transcripts. The purpose of this study was to compare the mRNA abundance of liver transporters and seek their novel transcripts during liver development. Livers from male C57BL/6J mice were collected at 12 ages from prenatal to adulthood. The transcriptome was determined by RNA-Seq, with transcript abundance estimated by Cufflinks. Among 498 known transporters, the ontogeny of 62 known critical xenobiotic transporters was examined in detail. The cumulati

SUBMITTER: Cui JY 

PROVIDER: S-EPMC3355312 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature