Islet1 derivatives in the heart are of both neural crest and second heart field origin.

ABSTRACT:

Rationale

Islet1 (Isl1) has been proposed as a marker of cardiac progenitor cells derived from the second heart field and is utilized to identify and purify cardiac progenitors from murine and human specimens for ex vivo expansion. The use of Isl1 as a specific second heart field marker is dependent on its exclusion from other cardiac lineages such as neural crest.

Objective

Determine whether Isl1 is expressed by cardiac neural crest.

Methods and results

We used an intersectional fate-mapping system using the RC::FrePe allele, which reports dual Flpe and Cre recombination. Combining Isl1(Cre/+), a SHF driver, and Wnt1::Flpe, a neural crest driver, with Rc::FrePe reveals that some Isl1 derivatives in the cardiac outflow tract derive from Wnt1-expressing neural crest pr

SUBMITTER: Engleka KA 

PROVIDER: S-EPMC3355870 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature