Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.

ABSTRACT:

Background

Generation of potent anti-HIV antibody responses in mucosal compartments is a potential requirement of a transmission-blocking HIV vaccine. HIV-specific, functional antibody responses are present in breast milk, and these mucosal antibody responses may play a role in protection of the majority of HIV-exposed, breastfeeding infants. Therefore, characterization of HIV-specific antibodies produced by B cells in milk could guide the development of vaccines that elicit protective mucosal antibody responses.

Methods

We isolated B cells from colostrum of an HIV-infected lactating woman with a detectable neutralization response in milk and recombinantly produced and characterized the resulting HIV-1 Envelope (Env)-specific monoclonal antibodies (mAbs).

Results

The

SUBMITTER: Friedman J 

PROVIDER: S-EPMC3356285 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature