A pilot trial assessing urinary gene expression profiling with an mRNA array for diabetic nephropathy.

ABSTRACT:

Background

The initiation and progression of diabetic nephropathy (DN) is complex. Quantification of mRNA expression in urinary sediment has emerged as a novel strategy for studying renal diseases. Considering the numerous molecules involved in DN development, a high-throughput platform with parallel detection of multiple mRNAs is needed. In this study, we constructed a self-assembling mRNA array to analyze urinary mRNAs in DN patients with aims to reveal its potential in searching novel biomarkers.

Methods

mRNA array containing 88 genes were fabricated and its performance was evaluated. A pilot study with 9 subjects including 6 DN patients and 3 normal controls were studied with the array. DN patients were assigned into two groups according to their estimate glomerular rate

SUBMITTER: Zheng M 

PROVIDER: S-EPMC3356359 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature