Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin.

ABSTRACT:

Background

To investigate the presence of metallo-β-lactamase (MBL) genes and the genetic environment of the New Delhi metallo-β-lactamase gene bla(NDM-1) in bacteria of food animal origin.

Methodology/principal findings

Gram-negative bacteria with low susceptibility to imipenem (MIC>8 µg/mL) were isolated from swab samples collected from 15 animal farms and one slaughterhouse in eastern China. These bacteria were selected for phenotypic and molecular detection of known MBL genes and antimicrobial susceptibility testing. For the bla(NDM-1) positive isolate, conjugation and transformation experiments were carried out to assess plasmid transfer. Southern blotting was conducted to localize the bla(NDM-1) genes, and DNA sequencing was performed to determine the sequences of bla(

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3356367 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature