MyoD-dependent regulation of NF-κB activity couples cell-cycle withdrawal to myogenic differentiation.

ABSTRACT:

Background

Mice lacking MyoD exhibit delayed skeletal muscle regeneration and markedly enhanced numbers of satellite cells. Myoblasts isolated from MyoD-/- myoblasts proliferate more rapidly than wild type myoblasts, display a dramatic delay in differentiation, and continue to incorporate BrdU after serum withdrawal.

Methods

Primary myoblasts isolated from wild type and MyoD-/- mutant mice were examined by microarray analysis and further characterized by cell and molecular experiments in cell culture.

Results

We found that NF-κB, a key regulator of cell-cycle withdrawal and differentiation, aberrantly maintains nuclear localization and transcriptional activity in MyoD-/- myoblasts. As a result, expression of cyclin D is maintained during serum withdrawal, inhibiting e

SUBMITTER: Parker MH 

PROVIDER: S-EPMC3356597 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature