NanoStringNorm: an extensible R package for the pre-processing of NanoString mRNA and miRNA data.

ABSTRACT:

Motivation

The NanoString nCounter Platform is a new and promising technology for measuring nucleic acid abundances. It has several advantages over PCR-based techniques, including avoidance of amplification, direct sequence interrogation and digital detection for absolute quantification. These features minimize aspects of experimental error and hold promise for dealing with challenging experimental conditions such as archival formalin-fixed paraffin-embedded samples. However, systematic inter-sample technical artifacts caused by variability in sample preservation, bio-molecular extraction and platform fluctuations must be removed to ensure robust data.

Results

To facilitate this process and to address these issues for NanoString datasets, we have written a pre-processing pac

SUBMITTER: Waggott D 

PROVIDER: S-EPMC3356845 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature