Isolated primate primordial follicles require a rigid physical environment to survive and grow in vitro.

ABSTRACT:

Background

In vitro follicle growth is a promising fertility preservation strategy in which ovarian follicles are cultured to produce mature and fertilization-competent oocytes. However, in primates, there has been limited success with in vitro follicle growth starting from primordial and primary follicles because adequate isolation methods and culture strategies have not been established. Understanding how to use primordial follicles for fertility preservation has significant implications because these follicles are the most abundant in the ovary, are found in all females and are fairly resistant to cryopreservation and chemotherapeutics.

Methods

In the primate ovary, primordial follicles are concentrated near the collagen-rich ovarian cortex. To obtain these follicles, we

SUBMITTER: Hornick JE 

PROVIDER: S-EPMC3357191 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature