Asymmetric chromatid segregation in cardiac progenitor cells is enhanced by Pim-1 kinase.

ABSTRACT:

Rationale

Cardiac progenitor cells (CPCs) in the adult heart are used for cell-based treatment of myocardial damage, but factors determining stemness, self-renewal, and lineage commitment are poorly understood. Immortal DNA strands inherited through asymmetric chromatid segregation correlate with self-renewal of adult stem cells, but the capacity of CPCs for asymmetric segregation to retain immortal strands is unknown. Cardioprotective kinase Pim-1 increases asymmetric cell division in vivo, but the ability of Pim-1 to enhance asymmetric chromatid segregation is unknown.

Objective

We aimed to demonstrate immortal strand segregation in CPCs and the enhancement of asymmetric chromatid distribution by Pim-1 kinase.

Methods and results

Asymmetric segregation is tracked by

SUBMITTER: Sundararaman B 

PROVIDER: S-EPMC3357328 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature