Bacterial delivery of large intact genomic-DNA-containing BACs into mammalian cells.

ABSTRACT: Efficient delivery of large intact vectors into mammalian cells remains problematical. Here we evaluate delivery by bacterial invasion of two large BACs of more than 150 kb in size into various cells. First, we determined the effect of several drugs on bacterial delivery of a small plasmid into different cell lines. Most drugs tested resulted in a marginal increase of the overall efficiency of delivery in only some cell lines, except the lysosomotropic drug chloroquine, which was found to increase the efficiency of delivery by 6-fold in B16F10 cells. Bacterial invasion was found to be significantly advantageous compared with lipofection in delivering large intact BACs into mouse cells, resulting in 100% of clones containing intact DNA. Furthermore, evaluation of expression of the human hyp

SUBMITTER: Cheung W 

PROVIDER: S-EPMC3357338 | biostudies-literature | 2012 Mar-Apr

REPOSITORIES: biostudies-literature