Evaluation of the cell viability of human Wharton's jelly stem cells for use in cell therapy.

ABSTRACT: Human umbilical cord Wharton's jelly stem cells (HWJSCs) are gaining attention as a possible clinical source of mesenchymal stem cells for cell therapy and tissue engineering due to their high accessibility, expansion potential, and plasticity. We employed a combination of highly sensitive techniques to determine the average cell viability levels and proliferation capabilities of 10 consecutive cell passages of cultured HWJSCs and then used RNA microarrays to identify genes associated with changes in cell viability levels. We found an initial decrease in cell viability from the first to the third cell passage followed by an increase until the sixth passage and a final decrease from the sixth to tenth cell passages. The highest cell viability levels corresponded to the fifth and sixth passa

SUBMITTER: Garzon I 

PROVIDER: S-EPMC3358099 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature