Comparative assessment of detergent-based protocols for mouse lung de-cellularization and re-cellularization.

ABSTRACT: Several different detergent-based methods are currently being explored for de-cellularizing whole lungs for subsequent use as three-dimensional scaffolds for ex vivo lung tissue generation. However, it is not yet clear which of these methods may provide a scaffold that best supports re-cellularization and generation of functional lung tissue. Notably, the detergents used for de-cellularization activate matrix metalloproteinases that can potentially degrade extracellular matrix (ECM) proteins important for subsequent binding and growth of cells inoculated into the de-cellularized scaffolds. We assessed gelatinase activation and the histologic appearance, protein composition, and lung mechanics of the end product scaffolds produced with three different detergent-based de-cellularization meth

SUBMITTER: Wallis JM 

PROVIDER: S-EPMC3358122 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature