Dataset Information

Multidrug-resistant Acinetobacter baumannii Harboring OXA-24 carbapenemase, Spain.

ABSTRACT: In February 2006, a patient colonized with a multidrug-resistant sequence type 56 Acinetobacter baumannii strain was admitted to a hospital in Madrid, Spain. This strain spread rapidly and caused a large outbreak in the hospital. Clinicians should be alert for this strain because its spread would have serious health consequences.

SUBMITTER: Acosta J

PROVIDER: S-EPMC3358182 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Multidrug-resistant Acinetobacter baumannii Harboring OXA-24 carbapenemase, Spain.

Acosta Joshi J Merino Maria M Viedma Esther E Poza Margarita M Sanz Francisca F Otero Joaquín R JR Chaves Fernando F Bou Germán G

Emerging infectious diseases 20110601 6

In February 2006, a patient colonized with a multidrug-resistant sequence type 56 Acinetobacter baumannii strain was admitted to a hospital in Madrid, Spain. This strain spread rapidly and caused a large outbreak in the hospital. Clinicians should be alert for this strain because its spread would have serious health consequences. ...[more]

PMID: 21749771

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Project description:PurposeA novel variant of OXA-23, named OXA-423, was identified in an Acinetobacter baumannii clinical isolate. The aim of this study was to analyse the resistance phenotype of OXA-423.MethodsThe A. baumannii strain WY-0713 was isolated from an intensive care unit patient. PCR was used to detect the bla OXA-23-like genes. Amplifying, cloning and sequencing were performed for the complete bla OXA-23-like. The novel bla OXA-423 and its ancestor bla OXA-23 were cloned into the expression vector pET-28b(+), and transformed into E. coli Rosetta (DE3) for antibiotic susceptibility testing. SDS-PAGE, modified Hodge test and CarbaNP test were used for detecting the expression of OXA-423 and OXA-23.ResultsPCR screening of A. baumannii WY-0713 was positive for bla OXA-23-like genes. Sequencing of the PCR product identiﬁed a novel bla OXA-23-like, named bla OXA-423 which encoding OXA-423. OXA-423 differed from OXA-23 by a crucial amino acid substitution (Val128Ala). The V128A substitution was located at the conserved active-site motifs SAV of OXA-23. Antibiotic susceptibility testing performed using isogenic E. coli showed that the MICs of E. coli Rosetta (pET-OXA-423) for penicillins and carbapenems were lower (reduced MICs 4-fold to 16-fold) than that of E. coli Rosetta (pET-OXA-23). The MICs of cefotaxime, ceftazidime and aztreonam for both transformants remained the same as the acceptor strain. Moreover, OXA-423 was slightly inhibited by sulbactam, clavulanic acid and tazobactam. SDS-PAGE analysis showed that OXA-423 and OXA-23 were conspicuously expressed. Modified Hodge test and CarbaNP test were positive demonstrated both of them were functional.ConclusionOXA-423, the first report of an amino acid substitution located at conserved active-site motifs of OXA-23, conferred lower MIC values of penicillins and carbapenems as compared with OXA-23, while without affecting the resistance proﬁles of expanded-spectrum cephalosporins and aztreonam.

Dataset Information

Multidrug-resistant Acinetobacter baumannii Harboring OXA-24 carbapenemase, Spain.

Publications

Multidrug-resistant Acinetobacter baumannii Harboring OXA-24 carbapenemase, Spain.

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