Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA.

ABSTRACT: Cell migration is of paramount importance to organism development and maintenance as well as multiple pathological processes, including cancer metastasis. The RhoGTPases Rac1 and RhoA are indispensable for cell migration as they regulate cell protrusion, cell-extracellular matrix (ECM) interactions and force transduction. However, the consequences of their activity at a molecular level within the cell remain undetermined. Using a combination of FRET, FRAP and biochemical analyses we show that the interactions between the focal adhesion proteins vinculin and paxillin, as well as the closely related family member Hic-5 are spatially and reciprocally regulated by the activity of Rac1 and RhoA. Vinculin in its active conformation interacts with either paxillin or Hic-5 in adhesions in response

SUBMITTER: Deakin NO 

PROVIDER: S-EPMC3358283 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature