The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells.

ABSTRACT: Although the significance of lysine modifications of core histones for regulating gene expression is widely appreciated, the mechanisms by which these modifications are incorporated at specific regulatory elements during cellular differentiation remains largely unknown. In our previous studies, we have shown that in developing myoblasts the Msx1 homeoprotein represses gene expression by influencing the modification status of chromatin at its target genes. We now show that genomic binding by Msx1 promotes enrichment of the H3K9me2 mark on repressed target genes via recruitment of G9a histone methyltransferase, the enzyme responsible for catalyzing this histone mark. Interaction of Msx1 with G9a is mediated via the homeodomain and is required for transcriptional repression and regulation of

SUBMITTER: Wang J 

PROVIDER: S-EPMC3358287 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature