Age-related maculopathy susceptibility 2 participates in the phagocytosis functions of the retinal pigment epithelium.

ABSTRACT:

Aim

Age-related macular degeneration (AMD) is a multifactorial disease and a prevalent cause of visual impairment in developed countries. Many studies suggest that age-related maculopathy susceptibility 2 (ARMS2) is a second major susceptibility gene for AMD. At present, there is no functional information on this gene. Therefore, the purpose of the present study was to detect the expression of ARMS2 in retinal pigment epithelium (RPE) cells and to investigate the effect of ARMS2 on the phagocytosis function of RPE cells.

Methods

Immunofluorescence and reverse transcriptase PCR were used to demonstrate the presence and location of ARMS2 in ARPE-19 (human retinal pigment epithelial cell line, ATCC, catalog No.CRL-2302) cells. siRNA was used to knock down ARMS2 mRNA, and the ef

SUBMITTER: Xu YT 

PROVIDER: S-EPMC3359023 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature