Substrate- and isoform-specific proteome stability in normal and stressed cardiac mitochondria.

ABSTRACT:

Rationale

Mitochondrial protein homeostasis is an essential component of the functions and oxidative stress responses of the heart.

Objective

To determine the specificity and efficiency of proteome turnover of the cardiac mitochondria by endogenous and exogenous proteolytic mechanisms.

Methods and results

Proteolytic degradation of the murine cardiac mitochondria was assessed by 2-dimensional differential gel electrophoresis and liquid chromatography-tandem mass spectrometry. Mitochondrial proteases demonstrated a substrate preference for basic protein variants, which indicates a possible recognition mechanism based on protein modifications. Endogenous mitochondrial proteases and the cytosolic 20S proteasome exhibited different substrate specificities.

Conclusions<

SUBMITTER: Lau E 

PROVIDER: S-EPMC3359149 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature