Identification and characterization of microRNAs in Phaseolus vulgaris by high-throughput sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs) are endogenously encoded small RNAs that post-transcriptionally regulate gene expression. MiRNAs play essential roles in almost all plant biological processes. Currently, few miRNAs have been identified in the model food legume Phaseolus vulgaris (common bean). Recent advances in next generation sequencing technologies have allowed the identification of conserved and novel miRNAs in many plant species. Here, we used Illumina's sequencing by synthesis (SBS) technology to identify and characterize the miRNA population of Phaseolus vulgaris.

Results

Small RNA libraries were generated from roots, flowers, leaves, and seedlings of P. vulgaris. Based on similarity to previously reported plant miRNAs,114 miRNAs belonging to 33 conserved miRNA families

SUBMITTER: Pelaez P 

PROVIDER: S-EPMC3359237 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature