Artificial polyploidy improves bacterial single cell genome recovery.

ABSTRACT:

Background

Single cell genomics (SCG) is a combination of methods whose goal is to decipher the complete genomic sequence from a single cell and has been applied mostly to organisms with smaller genomes, such as bacteria and archaea. Prior single cell studies showed that a significant portion of a genome could be obtained. However, breakages of genomic DNA and amplification bias have made it very challenging to acquire a complete genome with single cells. We investigated an artificial method to induce polyploidy in Bacillus subtilis ATCC 6633 by blocking cell division and have shown that we can significantly improve the performance of genomic sequencing from a single cell.

Methodology/principal findings

We inhibited the bacterial cytoskeleton protein FtsZ in B.subtilis with

SUBMITTER: Dichosa AE 

PROVIDER: S-EPMC3359284 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature