A predictive model of intein insertion site for use in the engineering of molecular switches.

ABSTRACT: Inteins are intervening protein domains with self-splicing ability that can be used as molecular switches to control activity of their host protein. Successfully engineering an intein into a host protein requires identifying an insertion site that permits intein insertion and splicing while allowing for proper folding of the mature protein post-splicing. By analyzing sequence and structure based properties of native intein insertion sites we have identified four features that showed significant correlation with the location of the intein insertion sites, and therefore may be useful in predicting insertion sites in other proteins that provide native-like intein function. Three of these properties, the distance to the active site and dimer interface site, the SVM score of the splice site cas

SUBMITTER: Apgar J 

PROVIDER: S-EPMC3359363 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature