Evolutionary and experimental assessment of novel markers for detection of Xanthomonas euvesicatoria in plant samples.

ABSTRACT:

Background

Bacterial spot-causing xanthomonads (BSX) are quarantine phytopathogenic bacteria responsible for heavy losses in tomato and pepper production. Despite the research on improved plant spraying methods and resistant cultivars, the use of healthy plant material is still considered as the most effective bacterial spot control measure. Therefore, rapid and efficient detection methods are crucial for an early detection of these phytopathogens.

Methodology

In this work, we selected and validated novel DNA markers for reliable detection of the BSX Xanthomonas euvesicatoria (Xeu). Xeu-specific DNA regions were selected using two online applications, CUPID and Insignia. Furthermore, to facilitate the selection of putative DNA markers, a customized C program was designed to

SUBMITTER: Albuquerque P 

PROVIDER: S-EPMC3359998 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature