Human papillomavirus type 18 E6 and E7 genes integrate into human hepatoma derived cell line Hep G2.

ABSTRACT:

Background and objectives

Human papillomaviruses have been linked causally to some human cancers such as cervical carcinoma, but there is very little research addressing the effect of HPV infection on human liver cells. We chose the human hepatoma derived cell line Hep G2 to investigate whether HPV gene integration took place in liver cells as well.

Methods

We applied PCR to detect the possible integration of HPV genes in Hep G2 cells. We also investigated the expression of the integrated E6 and E7 genes by using RT-PCR and Western blotting. Then, we silenced E6 and E7 expression and checked the cell proliferation and apoptosis in Hep G2 cells. Furthermore, we analyzed the potential genes involved in cell cycle and apoptosis regulatory pathways. Finally, we used in situ hybr

SUBMITTER: Ma T 

PROVIDER: S-EPMC3360009 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature