Simple, rapid and cost-effective method for high quality nucleic acids extraction from different strains of Botryococcus braunii.

ABSTRACT: This study deals with an effective nucleic acids extraction method from various strains of Botryococcus braunii which possesses an extensive extracellular matrix. A method combining freeze/thaw and bead-beating with heterogeneous diameter of silica/zirconia beads was optimized to isolate DNA and RNA from microalgae, especially from B. braunii. Eukaryotic Microalgal Nucleic Acids Extraction (EMNE) method developed in this study showed at least 300 times higher DNA yield in all strains of B. braunii with high integrity and 50 times reduced working volume compared to commercially available DNA extraction kits. High quality RNA was also extracted using this method and more than two times the yield compared to existing methods. Real-time experiments confirmed the quality and quantity of the inp

SUBMITTER: Kim BH 

PROVIDER: S-EPMC3360624 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature