MicroRNA-21 inhibits Serpini1, a gene with novel tumour suppressive effects in gastric cancer.

ABSTRACT:

Background

A thorough understanding of gastric cancer at the molecular level is urgently needed. One prominent oncogenic microRNA, miR-21, was previously reported to be upregulated in gastric cancer.

Methods

We performed an unbiased search for downstream messenger RNA targets of miR-21, based on miR-21 dysregulation, by using human tissue specimens and the MKN28 human gastric carcinoma cell line. Molecular techniques include microRNA microarrays, cDNA microarrays, qRT-PCR for miR and mRNA expression, transfection of MKN28 with miR-21 inhibitor or Serpini1 followed by Western blotting, cell cycle analysis by flow cytometry and luciferase reporter assay.

Results

This search identified Serpini1 as a putative miR-21 target. Luciferase assays demonstrated direct interactio

SUBMITTER: Yamanaka S 

PROVIDER: S-EPMC3360813 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature