Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote.

ABSTRACT:

Background

The centrosome has a well-established role as a microtubule organizer during mitosis and cytokinesis. In addition, it facilitates the union of parental haploid genomes following fertilization by nucleating a microtubule aster along which the female pronucleus migrates toward the male pronucleus. Stable associations between the sperm aster and the pronuclei are essential during this directed movement.

Results

Our studies reveal that the zebrafish gene futile cycle (fue) is required in the zygote for male pronucleus-centrosome attachment and female pronuclear migration. We show that fue encodes a novel, maternally-provided long form of lymphoid-restricted membrane protein (lrmp), a vertebrate-specific gene of unknown function. Both maternal lrmp messenger RNA (mRNA)

SUBMITTER: Lindeman RE 

PROVIDER: S-EPMC3360835 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature