Dendritic cell depletion and repopulation in the lung after irradiation and bone marrow transplantation in mice.

ABSTRACT: Dendritic cells (DCs) are essential for innate and adaptive immunity, but are purported to exhibit variable radiosensitivity in response to irradiation in various bone marrow transplantation (BMT) protocols. To address this controversy, we analyzed the magnitude of depletion and repopulation of both lung CD11b(pos) DC and CD103(pos) DC subsets in response to irradiation and BMT in a murine model. In our study, CD45.2(pos) donor bone marrow cells were transplanted into irradiated CD45.1(pos) recipient mice to examine the depletion of recipient DC subsets and the repopulation of donor DC subsets. We observed an apoptosis-mediated and necrosis-mediated depletion (> 90%) of the recipient CD103(pos) DC subset, and only a 50-60% depletion of recipient CD11b(pos) DCs from lung parenchymal tissue

SUBMITTER: Hahn I 

PROVIDER: S-EPMC3361352 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature