Effect of aging on 5-hydroxymethylcytosine in the mouse hippocampus.

ABSTRACT:

Purpose

Aging is believed to affect epigenetic marking of brain DNA with 5-methylcytosine (5mC) and possibly via the 5mC to 5-hydroxymethylcytosine (5hmC) conversion by TET (ten-eleven translocation) enzymes. We investigated the impact of aging on hippocampal DNA 5-hydroxymethylation including in the sequence of aging-susceptible 5-lipoxygenase (5-LOX) gene.

Methods

Hippocampal samples were obtained from C57BL6 mice. Cellular 5hmC localization was determined by immunofluorescence. The global 5mC and 5hmC contents were measured with the corresponding ELISA. The 5-LOX 5hmC content was measured using a glucosyltransferase/enzymatic restriction digest assay. TET mRNA was measured using qRT-PCR.

Results

Global hippocampal 5hmC content increased during aging as did the 5hmC

SUBMITTER: Chen H 

PROVIDER: S-EPMC3361533 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature