Oligoarray comparative genomic hybridization-mediated mapping of suppressor mutations generated in a deletion-biased mutagenesis screen.

ABSTRACT: Suppressor screens are an invaluable method for identifying novel genetic interactions between genes in the model organism Caenorhabditis elegans. However, traditionally this approach has suffered from the laborious and protracted process of mapping mutations at the molecular level. Using a mutagen known to generate small deletions, coupled with oligoarray comparative genomic hybridization (aCGH), we have identified mutations in two genes that suppress the lethality associated with a mutation of the essential receptor tyrosine kinase rol-3. First, we find that deletion of the Bicaudal-C ortholog, bcc-1, suppresses rol-3-associated lethality. Second, we identify several duplications that also suppress rol-3-associated lethality. We establish that overexpression of srap-1, a single gene pres

SUBMITTER: Jones MR 

PROVIDER: S-EPMC3362295 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature