ADP-ribosylation factor 6 expression and activation are reduced in myometrium in complicated pregnancies.

ABSTRACT:

Background

ARF6 (ADP-ribosylation factor 6) small GTP binding protein plays critical roles in actin cytoskeleton rearrangements and membrane trafficking, including internalisation of G protein coupled receptors (GPCR). ARF6 operates by cycling between GDP-bound (inactive) and GTP-bound (active) forms and is a potential regulator of GPCR-mediated uterine activity during pregnancy and labour. ARF6 contains very low intrinsic GTP binding activity and depends on GEFs (guanine nucleotide exchange factors) such as CYTH3 (cytohesin 3) to bind GTP. ARF6 and CYTH3 were originally cloned from human placenta, but there is no information on their expression in other reproductive tissues.

Methods

The expression of ARF6, ARF1, and CYTH1-4 was investigated by measuring mRNA (using RT-PCR)

SUBMITTER: Kanamarlapudi V 

PROVIDER: S-EPMC3364193 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature