ATP-sensitive potassium channels exhibit variance in the number of open channels below the limit predicted for identical and independent gating.

ABSTRACT: In small cells containing small numbers of ion channels, noise due to stochastic channel opening and closing can introduce a substantial level of variability into the cell's membrane potential. Negatively cooperative interactions that couple a channel's gating conformational change to the conformation of its neighbor(s) provide a potential mechanism for mitigating this variability, but such interactions have not previously been directly observed. Here we show that heterologously expressed ATP-sensitive potassium channels generate noise (i.e., variance in the number of open channels) below the level possible for identical and independent channels. Kinetic analysis with single-molecule resolution supports the interpretation that interchannel negative cooperativity (specifically, the presence

SUBMITTER: Choi KH 

PROVIDER: S-EPMC3364246 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature