Breast cancer 1 (BrCa1) may be behind decreased lipogenesis in adipose tissue from obese subjects.

ABSTRACT:

Context

Expression and activity of the main lipogenic enzymes is paradoxically decreased in obesity, but the mechanisms behind these findings are poorly known. Breast Cancer 1 (BrCa1) interacts with acetyl-CoA carboxylase (ACC) reducing the rate of fatty acid biosynthesis. In this study, we aimed to evaluate BrCa1 in human adipose tissue according to obesity and insulin resistance, and in vitro cultured adipocytes.

Research design and methods

BrCa1 gene expression, total and phosphorylated (P-) BrCa1, and ACC were analyzed in adipose tissue samples obtained from a total sample of 133 subjects. BrCa1 expression was also evaluated during in vitro differentiation of human adipocytes and 3T3-L1 cells.

Results

BrCa1 gene expression was significantly up-regulated in both om

SUBMITTER: Ortega FJ 

PROVIDER: S-EPMC3364252 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature